电离辐射通过转化生长因子-β-诱导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：阐述电磁辐射是否可通过转化生长因子-β（TGF-β）-酪氨酸的上皮-间质转化 (EMT)来有助于癌肝细胞的来袭移入。可用工业产值2Gy(60)Coγ线太阳光是从生物器官的6种癌肝细胞，记录与EMT相关的变化，这包括分别利用显微镜核心技术，细胞内质区别于方法有，免疫荧光核心技术，水痘试验和Transwell小室试验来观察并检查肝细胞分组织特征，EMT标记，来袭移入意志力等。运用于酶联免疫吸附法检查这些癌肝细胞当中TGF-β细胞内总体，利用比如说抑制剂SB431542来分析报告TGF-β频谱路当中在电磁辐射EMT当中的发挥作用。经过工业产值为2Gy太阳光的癌肝细胞当中存有间叶肝细胞的表达，与假太阳光分组远比其上皮标记减少，间叶肝细胞标记增高，同时其来袭转移意志力减慢，TGF-β细胞内总体也提高。全面性发现由A549电磁辐射发挥作用于的EMT可通过对TGF-β频谱抑制再次发生逆转。这些表明TGF-β酪氨酸的EMT在电磁辐射发挥作用于减慢癌肝细胞来袭转移意志力当中起着关键发挥作用。

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